Detection of Fermentative Variants with Tetrazolium

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Detection of Fermentative Variants with Tetrazolium.

While trying to develop methods for finding dehydrogenase mutants, we found tetrazolium (triphenyl tetrazolium chloride) to be useful for detecting fermentative variants. This indicator has the advantage that the nonfermenting variants are stained a deep red whereas the fermenters and the background are neutral. For example, cultures of Escherichia coli K-12 were spread on nutrient agar plates ...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1948

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.56.5.695-695.1948